Nucleic Acid and Protein

Protein–nucleic acid interactions

Nucleic acid - protein fingerprints. Novel protein classification based on nucleic acid - protein recognition

Protein chemistry uses protein description and classification based on molecular mass and isoelectric point as general features. Enzymes are also compared by enzymatic reaction constants, namely Km and kcat values. Proteins are also studied by binding to different oligonucleotides. Here we suggest a simple experimental method for such a comparison of DNA binding proteins, which we call “nucleic...

MS04 Protein-protein and Protein-Nucleic acid interactions

24 European Crystallographic Meeting, ECM24, Marrakech, 2007 Page s132 Acta Cryst. (2007). A63, s132 MS04 P14 Preparation and crystallization of rat natural killer cell receptor NKR-P1B Ondrej Vanek, Petr Kolenko, Vladimir Kopecky, Jr., Jan Dohnalek, Karel Bezouska Department of Biochemistry, Faculty of Science, Charles University in Prague, Hlavova 2030,CZ-12840 Prague, Czech Republic. Institu...

Nucleic Acid & Protein in the Microplate Format

The quantitation of nucleic acids and protein are measurements common to many applications in basic science and clinical research. Several different assays for either total protein or nucleic acids that can be performed in microplates are described. In addition, the fundamental differences between conventional tube-based instrumentation and microplate readers and how these differences relate to...

Thermodynamic Database for Protein-Nucleic Acid Interactions

Protein-nucleic acid interaction plays an essential role in the regulation of gene expression. Structural and binding information provides insight into the mechanism of protein-nucleic acid recognition. Although there are structural databases for protein-nucleic acid complexes, there exists no database for protein-nucleic acid binding. Thus, we have decided to develop the Thermodynamic Database...